primary human epidermal melanocytes  (PromoCell)

Journal: Scientific Reports

Article Title: Delphinidin upregulates microRNA-let-7b expression through family with sequence similarity 222 member B

doi: 10.1038/s41598-025-15588-3

Figure Lengend Snippet: Identification of molecules that regulate the growth inhibitory effects of melanoma cells by delphinidin. ( a ) Schematic of genetic screening for molecules that mediate the inhibition of melanoma cell proliferation by delphinidin. ( b ) Fam222B protein levels in melanoma cell lines and NHEMs were assessed using Western blot analysis ( n = 1). ( c and d ) Fam222B mRNA expression and protein levels were evaluated in B16 cells treated with delphinidin for 48 h ( n = 3). Data are presented as mean ± SEM. Statistical analysis was conducted using one-way ANOVA followed by Dunnett’s multiple comparison test.

Article Snippet: Mouse melanoma B16 cells, human melanoma cell lines (A375, Hs294t, and MeWo), and normal human epidermal melanocytes (NHEMs) were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Inhibition, Western Blot, Expressing, Comparison

Journal: bioRxiv

Article Title: Unbiased functional genetic screens reveal essential RNA modifications in human cancer and drug resistance

doi: 10.1101/2024.07.13.603368

Figure Lengend Snippet: (A) Principal component analysis (PCA) using log 2 fold change (FC) of 159 gRNAs in 18 cell lines originating from blood, plasma cells, epithelial cells or skin melanocytes at day 22 of the experiment. ( B ) Number of significantly (FDR<0.5; purple) depleted gRNAs in at least one cell line. ( C, D ) Total number (C) and list of RNA modifying proteins (RMPs) (D) corresponding to significantly (FDR<0.05) depleted gRNAs in the indicated cell lines. ( E ) Proportion of all (left) (n=150), essential (middle) (n=50) or non-essential (right) (n=100) RMPs showing weak or strong tissue-specific expression (Human Protein Atlas). ( F,G ) PCA using RMP expression (n=143) (F) or whole transcriptomes (G) (normalized log 2 FPKM) in 18 cell lines (n=3 sequencing reactions per cell line). ( H ) Heatmap of RMP-expression shown in (F) average over tissue or showing primary normal cells (NHEK, NHEM). ( I ) Averaged correlation distance of RMP-expression shown in (H) in cancer and normal cells. ( J ) Protein level of RMPs in the indicated cell lines grouped by gRNAs significantly (FDR<0.05) depleted in the indicated cell line (purple) or in any tested cell line (orange) or never significantly (FDR>0.05) depleted in any cell line (grey). Shown are 114 out of 159 proteins present in all cell lines. Box plots show minimum, first quartile, median, third quartile, and maximum (I).

Article Snippet: Primary melanocytes (NHEM) were kept in melanocyte growth medium M3 (PromoCell) with 1% PS.

Techniques: Expressing, Sequencing